Part:BBa_K222003:Design

Mid1 gene disrupted from 489 to 2021 nt

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

1 gaattcatat cagtgtccag gacctctacc caaggcctta catcccactg aaaaaccaaa

     61 tcgttctcag agatactcag tctatattcc caattttcag aggcgtcgga actggacaga

    121 ggttgctccg tagtggaatt cactagctca gcgaccacat aaagatttga atacgtggag

    181 gtagcgttcg agctagaaga ctgcactgga ctgatggcta atgcttggat gtagccatcc

    241 tgaaaaactg cactttgacc tatatttggg ttctctaaaa ccgtttcatt aaaagagtag

    301 tatattgtta aatccacatc tgatctattc atgggcattt ggcatatatt gccgctaata

    361 aacattaaca cttcatacgt gggagcaaac ccataaccgg aggcttctgc gtcaatggta

    421 aatacaaaac tgtctttttt gcccgctgtt atgttagata agatcggggt ccattcataa

    481 atgctatcc------------------------------------------

gggcagcgcg gtcattggcg

   2041 ccatcaaggc cagcttcgag aacgaaactg ttaaagccaa cgtataatcg acgcaaatat

   2101 gtatagatac aatatgtaca gaacaactgc attgtgcaat ataacaacat aacacaacgc

   2161 ccagaacgaa ataaaaaaaa taaaagaaat agatgaaagc attttcaatt tgcataccgg

   2221 aaaccgtaaa tcaattggcg tctagctaac aactgagaat gcgaatcgcc aaattgttac

   2281 agaaagtagc attccgttac gtgatctgta ctttaacctc ttggacgtaa agaatggcag

   2341 aactctggct ctagtgttct gcgaatgcca gatcgggaaa tatttcccaa aagggcaaca

   2401 gcggcacgaa caagaatttc gtgttaaaaa tcgcgtccgg cgcgaaattt ttcgcggagg

   2461 catgcgacgc aaaagcgact cgaaatgtcg ggagccaaat gaggctacaa ggctgtgggc

   2521 agattttggc gttattatgg agaataaaag gaatggtagc ttccatatag cgataggaaa

   2581 acatatataa ggtcaatagg cctacatggt aatgggattg ataagcttgt ccttaactct

   2641 tgtgtcttgg aattactatt gcgtacatca gcaatcatcc attgtcaaga acaaaaacga

   2701 taacaaaaac aatcaattat acaaataaca gtaaagtaat aaa

Source

It comes from a deletion of the Mid1 wt gene in Saccharomyces cerevisiae.